TRIzol is the trade name for the TRI reagent. This reagent is used for the extraction of RNA, although it is also possible to obtain DNA and proteins with few modifications to the protocol. TRIzol has the ability to intervene both in tissues of animal and plant origin, which is why it is very useful, hence its frequency in molecular biology laboratories.
TRIzol is composed of the following active ingredients: Phenol (which is very toxic and volatile), hydroxyquinoline (RNase inhibitor), thiocyanate, ammonium, guanidine thiocyanate and glycerol.
Regarding its conservation, it can be mentioned that TRIzol is very sensitive to light, therefore it is advisable to keep it in a container that is not transparent and on occasions it is recommended that it be wrapped in aluminum foil, for greater security. In the same way, as this reagent is volatile, it is necessary to keep it at a temperature lower than room temperature, thus avoiding its evaporation.
TRIzol has some physical characteristics that distinguish it: it is a bright pink or translucent substance, its smell is intense. TRIzol preserves the integrity of nucleic acids in the tissue homogenization process. It is also capable of breaking down cells or cellular elements.
It is important to point out that before using this product, it is advisable to read all the instructions on how to use it, as well as the measures to be taken in the event of an emergency. This product must be handled with great care as it can be very corrosive and irritating and can cause death. In the event that the product falls and splashes on the clothing of the user, it is recommended to remove the clothing as soon as possible, before the liquid touches the skin and in cases where this happens, the regulations establish washing the area with lots of water.
